![]() ![]() Gene expression analysis of individual cells enables characterization of heterogeneous and rare cell populations, yet widespread implementation of existing single-cell gene analysis techniques has been hindered due to limitations in scale, ease, and cost. Here, we present a novel microdroplet-based, one-step reverse-transcriptase polymerase chain reaction (RT-PCR) platform and demonstrate the detection of three targets simultaneously in over 100,000 single cells in a single experiment with a rapid read-out. Bioz Stars score: 86/100, based on 1 PubMed citations. Our customized reagent cocktail incorporates the bacteriophage T7 gene 2.5 protein to overcome cell lysate-mediated inhibition and allows for one-step RT-PCR of single cells encapsulated in nanoliter droplets. Premier Biosoft used beacon designer 2 0 software Used Beacon Designer 2 0 Software, supplied by Premier Biosoft, used in various techniques. CYP1A1 (Table 6.2) were designed using the Beacon Designer software (PREMIER Biosoft. Fluorescent signals indicative of gene expressions are analyzed using a probabilistic deconvolution method to account for ambient RNA and cell doublets and produce single-cell gene signature profiles, as well as predict cell frequencies within heterogeneous samples. studies of human intestinal development, disease, and drug design. We also developed a simulation model to guide experimental design and optimize the accuracy and precision of the assay. Using mixtures of in vitro transcripts and murine cell lines, we demonstrated the detection of single RNA molecules and rare cell populations at a frequency of 0.1%. From highly evolved design and analysis tools to custom bioinformatics. PREMIER Biosoft is a leading bioinformatics company offering software products and services to government agencies, pharmaceutical, and biotechnology companies and academia across the globe. SVZ neurogenesis and Tis21 pathways primers were designed by the software Beacon Designer 8. ![]() This low cost, sensitive, and adaptable technique will provide an accessible platform for high throughput single-cell analysis and enable a wide range of research and clinical applications. Partnering product: SimGlycan for glycan analysis. ![]()
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